Abstract
3-MCPD, 2-MCPD and glycidol, and especially their fatty acid esters, are process contaminants that are formed, for example, during the refining of edible oil and fats. At least some of the above-mentioned substances are classified as potentially carcinogenic to humans, a fact which has prompted the introduction of rules and regulations that specify tolerable daily intake values and maximum levels in edible oils. Different analytical methods are available for the determination of these compounds. These methods follow two different strategies: Direct determination or, more commonly, indirect differential determination of the contaminants.
This AppNote describes a solution for the fully automated determination of 3-MCPD, 2-MCPD and glycidol in edible oils based on the reliable indirect method DGF C-VI 18 (10), which is essentially identical to the ISO 18363-1 and AOCS Cd 29c-13 methods.
Keywords
3-MCPD, 2-MCPD, glycidol, edible oil, lab automation, GC-MS, GC-MS/MS, DGF C-VI 18 (10), mid-point backflush
