Comprehensive Analysis of Drugs of Abuse in Blood and Urine with Automated Disposable Pipette Extraction and HPLC/MS/MS


App Note 6/2009

The analysis of opiates in blood and urine was accomplished using automated Disposable Pipette Extraction (DPX) followed by LC/MS analysis. The automated extractions were performed in about 5 minutes using a GERSTEL MultiPurpose Sampler (MPS). The eluents were subsequently dried and reconstituted in solvent and injected into a HPLC/MS/MS instrument. Recoveries ranged from 60 to 85% for the opiates in blood, and recoveries ranged from 78 to 85% for the opiates in hydrolyzed urine. The %RSDs were lower than 6% for all analytes.