Application Notes

Application Notes

AppNote 247: Determination of PFAS in Food of Animal Origin using online SPE Cleanup and LC-MS/MS


In the work presented here, perfluorinated compounds were extracted from different food types (egg, fish and meat) using aQuEChERS-like approach followed by online-SPE cleanup and LC-MS/MS determination. In addition to validating the analysis method in accordance with the EU Guidance Document [1], matrix-based calibrations were compared to solvent-based calibrations to illustrate the matrix effects and demonstrate the cleanup efficiency. Online-SPE enables the injection of large amounts of sample extract, helping to reach the required quantification limits of 0.01 to 0.05 μg/kg.


Food analysis, LC-MS/MS, online-SPE, SPExos, PFAS