Application Notes

Application Notes

AppBrief 204: Completely Automated Extraction and Determination of Antibiotics in Eggs using a Robotic Autosampler and LC-MS/MS Platform

Abstract

Antibiotics are a class of antimicrobial veterinary drugs widely used to control disease in food producing animals including egg laying chickens. However, antibiotics can migrate into and accumulate in the egg of the chicken being treated. Eating eggs that contain antibiotic residues could provoke allergic reactions or lead to the development, or dominance of resistant bacterial strains, rendering treatment of human patients with antibiotics ineffective. It is therefore important to develop efficient screening methods for antibiotic residues in eggs intended for human consumption.

Extraction of antibiotic compounds from eggs can be challenging. Eggs are known to contain large amounts of lecithin, which is a mixture of phospholipids, and significant amounts of fats. It is important that the final egg extract be free of these co-extractive substances since they are known to lead to ion suppression and interference during LC/MS/MS analysis. Automating the entire process including liquid-liquid extraction of the raw egg sample, pass-through solid phase extraction to remove ion suppressing phospholipids, and analysis by LC/MS/MS provides the critical high throughput needed for the analysis for antibiotics in eggs.

Keywords

Sample Preparation, SPE, LC/MS/MS, High Throughput Lab Automation, Veterinary Drugs, Antibiotic