Optimized LC separation and MS detection – the best of both worlds
When developing LC/MS methods, we work hard to reach the goal of achieving the perfect LC separation and combining it with the most efficient ionization and lowest achievable MS detection limits for our analytes. In many cases, the LC separation may require a certain pH and polarity range of the eluent, while analyte ionization in the LC/MS ionization source requires yet another pH, a different buffer – or even derivatization of the analyte for the best possible efficiency or optimized spectral information.
The easiest and most efficient way to achieve an optimized method for both the liquid chromatography and the mass spectrometry detection is to use GERSTEL’s LC/MS Effluent Optimizer (LEO). This module enables automated additions of a solvent mixture, buffer solution, or reagent to the LC effluent ensuring that the LC separation can be performed under optimal conditions while also enabling maximum yield in the MS ionization process. LEO is compatible with most LC / MS systems and is easily in integrated into the Agilent LC-tower. The capillaries and components of the LEO option are made of PEEK and therefore bio-compatible.
Whether you are looking to perform pH adjustment or post-column derivatization, for method development or routine analysis, when you use LEO and the GERSTEL MAESTRO software you can easily and efficiently control all parameters as part of the overall LC/MS method.