Fully Automated Analysis of Doping Substances in Equine Urine by Disposable Pipette Extraction (DPX) coupled to GC/QqQ-MS

Abstract

This application note describes a fully automated method for the determination of selected doping compounds in equine urine. A GERSTEL MultiPurpose autosampler (MPS) with Disposable Pipette Extraction (DPX) option is employed for extraction and cleanup. After gas chromatographic separation the analytes are detected by a triple-quadrupole mass spectrometer (QqQ-MS). The method is rugged, provides an excellent cleanup of the complex sample matrix and shows very good limits of detection, from below 0.1 to just under 10 ng/mL, when compared to labor intensive manual manual SPE methods.