Automated Extraction of Aflatoxin B1 by Disposable Pipette Extraction from Corn followed by LC/MS/MS

Abstract

The extraction of Aflatoxin B1 residues from contaminated corn samples using disposable pipette extraction (DPX) is described. DPX is a solid-phase extraction (SPE) technique that is based on loosely contained sorbent inside a pipette tip fitted with a screen. This device provides faster extraction because only minimal conditioning steps are needed. A weak anion exchange sorbent (DPX-WAX) was found to provide selective extraction of Aflatoxin B1 from a crop sample. Recovery of the analyte of interest was 81 % with a relative standard deviation of 8 %.