Gerstel: Intelligent Automation for GC/MS and LC.MS

Automated QuEChERS Extraction


GERSTEL offers a wide range of QuEChERS Extraction Automation Options. Please see the application notes listed below to find the solution that best fits your needs.

Automated QuEChERS Extraction for Determination of Pesticide Residues in Foods using Gas Chromatography/Mass Spectrometry

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Keywords: Dispersive Solid Phase Extraction, Automation, QuEChERS, Pesticides

ABSTRACT

This paper describes an automated dispersive SPE (dSPE) cleanup method for QuEChERS extracts that is performed with commercially available kits from Agilent® Technologies. Extraction and clean-up is performed using a micro-scale version of this method and automation is achieved using a GERSTEL MPS autosampler equipped with an Anatune CF-100 centrifuge. The clean-up process is followed by automated injection of the cleaned extract to a GC/MS system using the GERSTEL Automated TDU-liner EXchange (ATEX) technique. The sensitivity and selectivity of GC/MS combined with the described injection technique, results in method detection limits that meet acceptance criteria for reporting maximum residue levels (MRLs) as established by regulatory agencies. The ability to automate the dSPE clean-up of QuEChERS extracts and to couple extraction and clean-up directly to GC-MS analysis, results in the improved laboratory productivity by streamlining the complete analytical process.


Automated Sample Preparation and Analysis Workflows for Pesticide Residue Screenings in Food Samples using DPX-QuEChERS with LC/MS/MS

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Keywords: Pesticide residue monitoring, QuEChERS, LC/MS/MS, Sample Preparation, DPX, Lab Automation

ABSTRACT

In this report, we describe an automated sample preparation and analysis workflow for the screening of pesticides residues in different food matrices (fruits, vegetables and spices) by LC/MS/MS. The automated cleanup of the QuEChERS extracts methodology was performed using disposable pipette extraction (DPX). Analytical methodology for confirming the presence of a variety of pesticides in various food samples was developed using a GERSTEL MultiPurpose Sampler (MPS), a combined autosampler and liquid handling robot, interfaced to an AB SCIEX QTRAP® 4500 LC/MS/MS System.


Automated QuEChERS Extraction for the Confirmation of Pesticide Residues in Foods using LC/MS/MS

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Keywords: QuEChERS, LC/MS/MS, Sample Preparation, Lab Automation

ABSTRACT

QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation methods were developed to help monitor pesticides in a range of food samples [1]. These methods are quite labor intensive, however, since they include several manual steps, such as shaking, centrifugation, and dispersive SPE. If dispersive SPE clean up of QuEChERS type extracts could be automated, laboratory productivity for monitoring pesticide residues in food could be improved significantly. In the work presented here, an automated dispersive SPE clean-up method for QuEChERS extracts was developed and combined with LC/MS/MS analysis of the cleaned extracts. Extracts were prepared with commercially available kits from Agilent Technologies. Automation was achieved using a GERSTEL MPS XL PrepStation configured with an Anatune CF-100 centrifuge. Analytical methodology for confirming the presence of a variety of pesticides in a range food samples was developed using an Agilent G6460A Triple Quadrupole Mass Spectrometer. The sensitivity and selectivity of LC/MS/MS enable sufficiently low limits of determination to meet acceptance criteria for reporting the maximum residue levels (MRLs) that are established by regulatory agencies. The ability to automate the dispersive SPE clean-up of QuEChERS extracts combined with introduction of the cleaned extract directly to the LC/MS/MS system results in improved laboratory productivity by streamlining the complete analytical process.


Automated QuEChERS Extraction for the Confirmation of Pesticide Residues in Foods using LC/MS/MS

DOWNLOADS

Keywords: QuEChERS, LC/MS/MS, Sample Preparation, Lab Automation

ABSTRACT

QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation methods were developed to help monitor pesticides in a range of food samples [1]. These methods are quite labor intensive, however, since they include several manual steps, such as shaking, centrifugation, and dispersive SPE. If dispersive SPE clean up of QuEChERS type extracts could be automated, laboratory productivity for monitoring pesticide residues in food could be improved significantly. In the work presented here, an automated dispersive SPE clean-up method for QuEChERS extracts was developed and combined with LC/MS/MS analysis of the cleaned extracts. Extracts were prepared with commercially available kits from Agilent Technologies. Automation was achieved using a GERSTEL MPS XL PrepStation configured with an Anatune CF-100 centrifuge. Analytical methodology for confirming the presence of a variety of pesticides in a range food samples was developed using an Agilent G6460A Triple Quadrupole Mass Spectrometer. The sensitivity and selectivity of LC/MS/MS enable sufficiently low limits of determination to meet acceptance criteria for reporting the maximum residue levels (MRLs) that are established by regulatory agencies. The ability to automate the dispersive SPE clean-up of QuEChERS extracts combined with introduction of the cleaned extract directly to the LC/MS/MS system results in improved laboratory productivity by streamlining the complete analytical process.